WebJul 10, 2024 · Silver-stained SDS PAGE gel of IP proteins showing chicken CTCF protein of approximately 140 kDa, and lamprey CTCF protein of >250 kDa. Detailed procedures of western blotting and ... WebCUT&RUN was performed on 500k K562 cells with either CTCF (0.125 µg) or IgG negative control (0.5 µg, EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v3.0 (EpiCypher 14-1048).Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit …
CTCF Antibody - ChIP-seq grade (C15410210) Diagenode
WebAbFlex® CTCF recombinant antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of MCF7 chromatin and 4 µg of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to … WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. pom pom pictures craft kit
Analysis of absolute amounts of two meiotic cohesin subunits, …
WebCCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers.1 It was first identified as a transcriptional repressor of the chicken c-myc gene, a regulatory gene that encodes the WebApr 6, 2012 · Here, we report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses. We found that stimulation of human corneal … WebSep 15, 2009 · Purpose: There is compelling evidence of a relationship between poly(ADP-ribosyl)ation and tumorigenesis; however, much less is known about the role of specific targets of poly(ADP-ribosyl)ation in tumor development. Two forms of the multifunctional transcription factor, CTCF, were previously identified: a 130-kDa protein (CTCF-130), … shannon wright benoit houston tx