High-density oligonucleotide arrays

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WebWe have developed an approach that is based on hybridization to small, high-density arrays containing tens of thousands of synthetic oligonucleotides. The arrays are … Web1 de set. de 2011 · With flexible sample preparation, high-density oligonucleotide arrays can be tailored for even larger scale genome-wide SNP discovery as well as validation. Show less See publication ...

comparison of normalization methods for high density …

WebIn this paper we report exploratory analyses of high-density oligonucleotide array data from the Affymetrix GeneChip system with the objective of improving upon … Web7 de fev. de 2007 · High-density DNA probe arrays provide a massively parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research and diagnostics. Light-directed combinatorial oligonucleotide synthesis has enabled the large-scale production of GeneChip® probe arrays which contain several hundred of thousand … io psychology programs oregon

DNA microarrays and their applications in medical microbiology

Web1 de jan. de 1997 · Oligonucleotide arrays are also used for DNA sequencing and genotyping (Gingeras et al., 1998), which is a promising application of the high-density oligonucleotide hybridization platform. ... Web22 de jan. de 2003 · Request PDF A Comparison of Normalization Methods for High Density Oligonucleotide Array Data Based on Variance and Bias Motivation: When running experiments that involve multiple high density ... WebAn approach in which sequence information is used directly to design high–density, two–dimensional arrays of synthetic oligonucleotides is developed, which have been … iopsys icwmp

High-density oligonucleotide array analysis reveals extensive ...

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http://bmbolstad.com/misc/normalize/bolstad_norm_paper.pdf Web23 de dez. de 2002 · We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E 2) on the transcriptomes from the medial basal hypothalamus and other brain regions of E 2-treated (10 μg) adult female mice. Our results have revealed several unanticipated gene regulations. on the plains of hesitationWeb26 de nov. de 1996 · High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for … i/o psychology research

"WebTo do so, high-density DNA microarrays are fabricated on a base-sensitive monomer that allows the DNA to be conveniently cleaved and retrieved after synthesis and deprotection. The fabrication protocol is optimized so as to limit the number of synthetic errors and to that effect, a layer of β-carotene solution is introduced to absorb UV photons that may … " - High-density oligonucleotide arrays

High-density oligonucleotide arrays

PHOTOLITHOGRAPHIC SYNTHESIS OF HIGH-DENSITY OLIGONUCLEOTIDE PROBE ARRAYS

WebHigh-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. … Web22 de jan. de 2003 · Motivation: When running experiments that involve multiple high density oligonucleotide arrays, it is important to remove sources of variation between arrays of non-biological origin. Normalization is a process for reducing this variation.

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WebHepatic NK cells are more cytotoxic than blood NK cells against tumor cells. To understand the basis of this difference in cytotoxicity we analyzed RNA derived from freshly isolated … WebAbstract. Motivation: High-density oligonucleotide arrays (GeneChip, Affymetrix, Santa Clara, CA) have become a standard research tool in many areas of biomedical …

WebAlthough oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a specific technique of manufacturing. … Web1 de dez. de 1996 · Hacia, J., Brody, L., Chee, M. et al. Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysis. Nat Genet 14 , 441–447 (1996 ...

WebHigh-density oligonucleotide arrays have been shown to be well suited to this task11–14, allowing the simultane-ous monitoring of all yeast genes15–17, all Escherichia coligenes, tens of thousands of human and mouse genes and selective sub-sets of genes from a wide range of organisms. Oligonucleotide arrays for expression monitoring are ... Web1 de dez. de 1996 · We have designed high–density arrays consisting of over 96,600 oligonucleotides 20–nucleotides (nt) in length to screen for a wide range of heterozygous …

Web1 de jan. de 2002 · High-density oligonucleotide arrays (gene chips): The probe is generated in situ on the surface of the matrix. The leader in these arrays is Affymetrix …

WebAbstract: Genomic DNA-based probe selection by using high density oligonucleotide arrays has recently been applied to heterologous species (Xspecies). With the advent of this new approach, researchers are able to study the genome and transcriptome of a non-model iop talk physics iopsys software solutionsWeb1 de jan. de 2002 · The leading arrays in the category of high-density oligonucleotide arrays are manufactured by Affymetrix and utilize the combinatorial synthesis principle. 9 The arrays are designed by using a light-directed chemical synthesis process that creates a series of photolithographic masks to define chip exposure sites, followed by specific … on the places you will goWeb24 de mai. de 1994 · These probe arrays, or DNA chips, can then be applied to parallel DNA hybridization analysis, directly yielding sequence information. In a preliminary experiment, a 1.28 x 1.28 cm array of 256 different octanucleotides was produced in 16 chemical reaction cycles, requiring 4 hr to complete. The hybridization pattern of … iop teaching electricityWeb24 de jul. de 2015 · The methods for preparing DNA chips combine photochemistry and photolithography with solid-phase DNA synthesis chemistry to generate a high-density oligonucleotide probe array in situ [6,7,8]. These two-dimensional arrays containing hundreds or thousands of oligonucleotide probes provide a powerful DNA sequence … on the plains photographyWeb19 de set. de 2003 · High-density oligonucleotide arrays (1, 2) have revolutionized the study of gene expression.The technology enables researchers to detect and quantify tens of thousands of transcripts in a single experiment and has become a standard for the discovery of gene functions, drug evaluation, pathway dissection, and classification of clinical … on the plains of hopeWeb1 de jan. de 1996 · Refereed paperHigh-density oligonucleotide arrays. We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a … i o psychology phd online