Phospho-mtor antibody

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August undefined, 2024

Webtorc-specific phosphorylation of mtor phospho-ser2481 does is a marker for intact mtorc2.pdf. 2016-06-15 ... WebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR (7C10) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed line).

Phospho-Specific Antibodies Bio-Rad

WebAnti-mTOR (phospho T2446) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho T2446) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse Immunogen Synthetic peptide corresponding to Human mTOR (phospho T2446). Database link: P42345 General notes WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. shs teachers

Phospho-AKT / GSK3 beta / mTor ELISA kit (ab279732) Abcam

WebAn antibody to the phosphorylated S6 ribosomal protein was used to stain the tumors, all high-grade sarcomas. Pretreatment biopsy or resection material was tested: the original tumor (n=6) or tumor recurrence/metastasis (n=14); either of these may have been after treatment with other agents. Web80596-1-RR targets Phospho-mTOR (Ser2448) in WB, ELISA applications and shows reactivity with Human, Rat samples. Background Information MTOR, also named as … WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 ... theory treeca blazer

mTOR Antibody SCBT - Santa Cruz Biotechnology

Anti-phospho-mTOR (Thr2446) Antibody - Sigma-Aldrich

WebRabbit recombinant monoclonal mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free. Validated in WB, IHC, Dot and tested in Mouse, Human. WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … shs technical schoolWebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein. theory treeca linen

"WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... " - Phospho-mtor antibody

Phospho-mtor antibody

Phospho-mTOR (Ser2481) Polyclonal Antibody - Thermo Fisher …

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR426 (2)] to mTOR (phospho S2448) - BSA and Azide free. Suitable for: IHC-P, … WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl …

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WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours.

WebPolyclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1872 publications. Validated for Western Blotting, Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-mTOR (Ser2448) Antibody (CST #2971) is ready to … WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume.

WebDescription: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. … WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the …

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold

WebImmunofluorescent analysis of Phospho-mTOR (Ser2481) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X … shs teamWebtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ... shst employeesWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold sh stellenportalWebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot. - Find MSDS or SDS, a COA, data sheets and more information. theory treeca pull on pantsWebindicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. mTOR Antibodies Phospho-Specific mTOR Antibodies mTOR CRISPR Knockout, HDR and Nickase Knockout Plasmids mTOR CRISPR Activation Plasmids mTOR siRNA, shRNA Plasmid and shRNA Lentiviral Particles gene silencers shs telefonWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. shs telehealthWebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … shstheatre.com